No, the trial version is 64 bit compatable.
http://www.symantec.com/business/products/sysreq.jsp?pcid=pcat_info_risk_comp&pvid=endpt_prot_1
System Requirements
Client Workstations and Servers
Processor
* Note: Itanium is not supported
* Intel Pentium processor or compatible architecture (32-bit and 64-bit)
Windows Operating Systems (32-bit and 64-bit versions)
* Windows 2000 Professional, Server, Advanced Server, Datacenter Server, Small Business Server with Service Pack 3 or later
* Windows XP Home Edition, Professional Edition, Tablet PC Edition, Embedded Edition, Media Center Edition with Service Pack 1 or later
* Windows Server 2003 Standard Edition, Enterprise Edition, Datacenter Edition, Web Edition, Small Business Server, Compute Cluster Server, Storage Server
* Windows Vista Home Basic, Home Premium, Business, Enterprise, Ultimate
* Windows Server 2008 Standard Edition, Enterprise Edition, Datacenter Edition, Web Edition
* Windows Small Business Server, Standard Edition, Premium Edition
* Windows Essential Business Server, Standard Edition, Premium Edition